cat 03223 (HyTest)

HyTest cat 03223
Cat 03223, supplied by HyTest, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cat+03223/us10376881-444-33-42?v=HyTest
Average 94 stars, based on 1 article reviews

cat 03223 - by Bioz Stars, 2026-07

94/100 stars

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antibodies against otub2 (Novus Biologicals)

Novus Biologicals antibodies against otub2

Colonic expression <t>OTUB2</t> is downregulated in colitis. (A) Representative immunohistochemical staining of OTUB2 in control and UC colon samples. Scale bar = 50 µm. (B) Representative immunohistochemical staining of OTUB2 in colon samples from control and DSS‐treated C57BL/6 mice. Control mice received regular drinking water. Mice in the DSS group were given 2% DSS for 8 days, followed by regular drinking water for 2 days. Scale bar = 50 µm. (C, D) Representative immunoblots (C) and relative quantification (D) of OTUB2 protein in colon samples from control and DSS‐treated C57BL/6 mice. (E) Relative mRNA levels of Otub2 in colon samples from control and DSS‐treated C57BL/6 mice were analysed by qRT‐PCR. (F) Western blot analysis of OTUB2 protein abundance in colon samples from Otub2 +/+ and Otub2 –/– mice. (G) The representative image (left) and length (right) of colons from Otub2 +/+ and Otub2 –/– mice. (H, I) Representative H&E (H) and PAS/AB (I) staining of colons from Otub2 +/+ and Otub2 –/– mice. Scale bar = 50 µm. Data in D, E and G are shown as mean ± SEM. ns, no significant difference. * p < .05, ** p < .01.

Antibodies Against Otub2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cat+03223/pmc11446981-67-16-21?v=Novus+Biologicals
Average 94 stars, based on 1 article reviews

antibodies against otub2 - by Bioz Stars, 2026-07

94/100 stars

Buy from Supplier

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Colonic expression OTUB2 is downregulated in colitis. (A) Representative immunohistochemical staining of OTUB2 in control and UC colon samples. Scale bar = 50 µm. (B) Representative immunohistochemical staining of OTUB2 in colon samples from control and DSS‐treated C57BL/6 mice. Control mice received regular drinking water. Mice in the DSS group were given 2% DSS for 8 days, followed by regular drinking water for 2 days. Scale bar = 50 µm. (C, D) Representative immunoblots (C) and relative quantification (D) of OTUB2 protein in colon samples from control and DSS‐treated C57BL/6 mice. (E) Relative mRNA levels of Otub2 in colon samples from control and DSS‐treated C57BL/6 mice were analysed by qRT‐PCR. (F) Western blot analysis of OTUB2 protein abundance in colon samples from Otub2 +/+ and Otub2 –/– mice. (G) The representative image (left) and length (right) of colons from Otub2 +/+ and Otub2 –/– mice. (H, I) Representative H&E (H) and PAS/AB (I) staining of colons from Otub2 +/+ and Otub2 –/– mice. Scale bar = 50 µm. Data in D, E and G are shown as mean ± SEM. ns, no significant difference. * p < .05, ** p < .01.

Journal: Clinical and Translational Medicine

Article Title: Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation

doi: 10.1002/ctm2.70038

Figure Lengend Snippet: Colonic expression OTUB2 is downregulated in colitis. (A) Representative immunohistochemical staining of OTUB2 in control and UC colon samples. Scale bar = 50 µm. (B) Representative immunohistochemical staining of OTUB2 in colon samples from control and DSS‐treated C57BL/6 mice. Control mice received regular drinking water. Mice in the DSS group were given 2% DSS for 8 days, followed by regular drinking water for 2 days. Scale bar = 50 µm. (C, D) Representative immunoblots (C) and relative quantification (D) of OTUB2 protein in colon samples from control and DSS‐treated C57BL/6 mice. (E) Relative mRNA levels of Otub2 in colon samples from control and DSS‐treated C57BL/6 mice were analysed by qRT‐PCR. (F) Western blot analysis of OTUB2 protein abundance in colon samples from Otub2 +/+ and Otub2 –/– mice. (G) The representative image (left) and length (right) of colons from Otub2 +/+ and Otub2 –/– mice. (H, I) Representative H&E (H) and PAS/AB (I) staining of colons from Otub2 +/+ and Otub2 –/– mice. Scale bar = 50 µm. Data in D, E and G are shown as mean ± SEM. ns, no significant difference. * p < .05, ** p < .01.

Article Snippet: Subsequently, the samples were transferred to PVDF membranes (Cat#: 10600023, Cytiva), followed by incubation with primary antibodies against OTUB2 (Cat#: NBP2‐03223, Novus Biologicals), OTUB1 (Cat#: NBP1‐49934, Novus Biologicals), NOD2 (Cat #: sc‐56168, Santa Cruz Biotechnology), p‐RIPK2 (Cat#: 14397, Cell Signaling Technology), RIPK2 (Cat#: 4142S, Cell Signaling Technology), XIAP (CAT#: 10037‐1‐Ig, Proteintech), p‐p65 (Cat#: 3033S, Cell Signaling Technology), p65 (Cat#: 8242S, Cell Signaling Technology), p‐JNK (Cat#: 4668S, Cell Signaling Technology), JNK (Cat#: 9252S, Cell Signaling Technology) Technology), p‐p38 (Cat#: 4631S, Cell Signaling Technology), p38 (Cat#: 8690S, Cell Signaling Technology), HA (Cat#: 51064‐2‐AP, Proteintech), FLAG (Cat#: 14793S, Cell Signaling Technology), MYC (Cat#: 16286‐1‐AP, Proteintech), K48 polyubiquitin (Cat#: 8081S, Cell Signaling Technology), or GAPDH (Cat#:60004‐1‐Ig, Proteintech) at 4°C overnight.

Techniques: Expressing, Immunohistochemical staining, Staining, Control, Western Blot, Quantitative Proteomics, Quantitative RT-PCR

OTUB2 ablation exacerbates DSS‐induced colonic inflammation. (A, B) Otub2 +/+ and Otub2 –/– mice were fed drinking water containing 2% DSS for 8 days, and then given normal drinking water for 2 days. Body weight (A) and disease activity index (B) were recorded daily ( n = 6/group). (C, D) The representative image (C) and length (D) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. (E) Representative TUNEL staining of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. Scale bar = 50 µm. (F, G) Histology score (F) as well as representative H&E and PAS/AB staining (G) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. Scale bar = 50 µm. (H) The colonic transcription levels of Ilb , Il6 , Tnf , Cxcl2 and Cxcl10 mRNA were analysed by qRT‐PCR. Data are presented as the relative increase over untreated control samples. Data in A, B, D, E and G are shown as mean ± SEM. * p < .05, ** p < .01, *** p < .001.

Journal: Clinical and Translational Medicine

Article Title: Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation

doi: 10.1002/ctm2.70038

Figure Lengend Snippet: OTUB2 ablation exacerbates DSS‐induced colonic inflammation. (A, B) Otub2 +/+ and Otub2 –/– mice were fed drinking water containing 2% DSS for 8 days, and then given normal drinking water for 2 days. Body weight (A) and disease activity index (B) were recorded daily ( n = 6/group). (C, D) The representative image (C) and length (D) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. (E) Representative TUNEL staining of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. Scale bar = 50 µm. (F, G) Histology score (F) as well as representative H&E and PAS/AB staining (G) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. Scale bar = 50 µm. (H) The colonic transcription levels of Ilb , Il6 , Tnf , Cxcl2 and Cxcl10 mRNA were analysed by qRT‐PCR. Data are presented as the relative increase over untreated control samples. Data in A, B, D, E and G are shown as mean ± SEM. * p < .05, ** p < .01, *** p < .001.

Techniques: Activity Assay, TUNEL Assay, Staining, Quantitative RT-PCR, Control

Deficiency of OTUB2 in haematopoietic cells exacerbates DSS‐induced colitis. (A) Experimental flowchart for bone marrow transplantation. (B) Eight weeks after bone marrow transplantation, OTUB2 expression in splenocytes of Otub2 +/+ mice receiving bone marrow from Otub2 +/+ (WT→WT) and Otub2 –/– (KO→WT) mice was analysed by Western blot. (C, D) The chimeric mice were fed drinking water containing 2% DSS for 8 days, and then given normal drinking water for 2 days. Body weight (C) and disease activity index (D) were recorded daily ( n = 7/group). (E, F) The representative image (E) and length (F) of colons from chimeric mice on day 10 after DSS treatment. (G and H) Histology score (G) as well as representative H&E and PAS/AB staining (H) of colons from chimeric mice on day 10 after DSS treatment. Scale bar = 50 µm. Data in C, D, F and G are shown as mean ± SEM. * p < .05, ** p < .01, *** p < .001.

Journal: Clinical and Translational Medicine

Article Title: Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation

doi: 10.1002/ctm2.70038

Figure Lengend Snippet: Deficiency of OTUB2 in haematopoietic cells exacerbates DSS‐induced colitis. (A) Experimental flowchart for bone marrow transplantation. (B) Eight weeks after bone marrow transplantation, OTUB2 expression in splenocytes of Otub2 +/+ mice receiving bone marrow from Otub2 +/+ (WT→WT) and Otub2 –/– (KO→WT) mice was analysed by Western blot. (C, D) The chimeric mice were fed drinking water containing 2% DSS for 8 days, and then given normal drinking water for 2 days. Body weight (C) and disease activity index (D) were recorded daily ( n = 7/group). (E, F) The representative image (E) and length (F) of colons from chimeric mice on day 10 after DSS treatment. (G and H) Histology score (G) as well as representative H&E and PAS/AB staining (H) of colons from chimeric mice on day 10 after DSS treatment. Scale bar = 50 µm. Data in C, D, F and G are shown as mean ± SEM. * p < .05, ** p < .01, *** p < .001.

Techniques: Transplantation Assay, Expressing, Western Blot, Activity Assay, Staining

OTUB2 enhances MDP‐induced cytokine production in macrophages. (A, B) Representative OTUB2 (red) and F4/80 (green) immunofluorescence staining of colon samples from humans (A) and mice (B). Scale bar = 100 µm. (C, D) BMDM isolated from C57BL/6 mice were stimulated with 500 ng/mL LPS (C) or 200 ng/mL L18‐MDP (D) for indicated periods of time. OTUB2 protein levels were then analysed by Western blot. (E) BMDM isolated from C57BL/6 mice were stimulated with 200 ng/mL L18‐MDP for indicated periods of time. The relative mRNA levels of Otub2 were determined by qRT‐PCR. Data are presented as the relative increase over untreated control samples. (F) RAW264.7 cells were stimulated with 200 ng/mL L18‐MDP for indicated periods of time. OTUB2 protein levels were then analysed by Western blot. (G–K) BMDMs isolated from Otub2 +/+ and Otub2 –/– mice were stimulated with 200 ng/mL L18‐MDP for 3 h or left untreated. The relative expression of Ilb (G), Il6 (H), Tnf (I), Cxcl2 (J) and Cxcl10 (K) mRNA was determined by qRT‐PCR. Data are presented as the relative increase over untreated control samples. Data in E and G–K are displayed as mean ± SEM. * p < .05, ** p < .01, *** p < .001.

Journal: Clinical and Translational Medicine

Article Title: Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation

doi: 10.1002/ctm2.70038

Figure Lengend Snippet: OTUB2 enhances MDP‐induced cytokine production in macrophages. (A, B) Representative OTUB2 (red) and F4/80 (green) immunofluorescence staining of colon samples from humans (A) and mice (B). Scale bar = 100 µm. (C, D) BMDM isolated from C57BL/6 mice were stimulated with 500 ng/mL LPS (C) or 200 ng/mL L18‐MDP (D) for indicated periods of time. OTUB2 protein levels were then analysed by Western blot. (E) BMDM isolated from C57BL/6 mice were stimulated with 200 ng/mL L18‐MDP for indicated periods of time. The relative mRNA levels of Otub2 were determined by qRT‐PCR. Data are presented as the relative increase over untreated control samples. (F) RAW264.7 cells were stimulated with 200 ng/mL L18‐MDP for indicated periods of time. OTUB2 protein levels were then analysed by Western blot. (G–K) BMDMs isolated from Otub2 +/+ and Otub2 –/– mice were stimulated with 200 ng/mL L18‐MDP for 3 h or left untreated. The relative expression of Ilb (G), Il6 (H), Tnf (I), Cxcl2 (J) and Cxcl10 (K) mRNA was determined by qRT‐PCR. Data are presented as the relative increase over untreated control samples. Data in E and G–K are displayed as mean ± SEM. * p < .05, ** p < .01, *** p < .001.

Techniques: Immunofluorescence, Staining, Isolation, Western Blot, Quantitative RT-PCR, Control, Expressing

OTUB2 enhances MDP‐induced signalling by inhibiting the proteasomal degradation of RIPK2. (A) After stimulation with 200 ng/mL L18‐MDP for indicated periods of time, BMDMs derived from Otub2 +/+ and Otub2 –/– mice were lysed and analysed by Western blot with indicated antibodies. Densitometric quantification is shown in the figure. (B) Schematic diagram of MDP‐induced signalling. (C) The protein abundance of NOD2, RIPK2 and XIAP in Otub2 +/+ and Otub2 –/– BMDMs was determined by Western blot. (D–F) The relative protein levels of NOD2 (D), XIAP (E) and RIPK2 (F) in Otub2 +/+ and Otub2 –/– BMDMs. (G) The relative mRNA levels of Ripk2 in Otub2 +/+ and Otub2 –/– BMDMs were determined by qRT‐PCR. (H) RAW264.7 cells were transfected with FLAG‐Vector or FLAG‐OTUB2 plasmids for 24 h. Thereafter, cells were lysed and analysed by Western blot with indicated antibodies. (I) After treatment with 20 ng/mL CHX for indicated periods of time, BMDMs isolated from Otub2 +/+ and Otub2 –/– mice were lysed and analysed by Western blot with indicated antibodies. (J, K) BMDMs isolated from Otub2 +/+ and Otub2 –/– mice were treated with 20 µM MG132 (J) or 50 µM CQ (K) for 6 h or left untreated. Whole Cell lysates were analysed by Western blot with indicated antibodies. Representative immunoblots (upper panel) and quantification (lower panel) are shown. Data in D–G, J and K are shown as mean ± SEM. ns, no significant difference. * p < .05, ** p < .01.

Journal: Clinical and Translational Medicine

Article Title: Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation

doi: 10.1002/ctm2.70038

Figure Lengend Snippet: OTUB2 enhances MDP‐induced signalling by inhibiting the proteasomal degradation of RIPK2. (A) After stimulation with 200 ng/mL L18‐MDP for indicated periods of time, BMDMs derived from Otub2 +/+ and Otub2 –/– mice were lysed and analysed by Western blot with indicated antibodies. Densitometric quantification is shown in the figure. (B) Schematic diagram of MDP‐induced signalling. (C) The protein abundance of NOD2, RIPK2 and XIAP in Otub2 +/+ and Otub2 –/– BMDMs was determined by Western blot. (D–F) The relative protein levels of NOD2 (D), XIAP (E) and RIPK2 (F) in Otub2 +/+ and Otub2 –/– BMDMs. (G) The relative mRNA levels of Ripk2 in Otub2 +/+ and Otub2 –/– BMDMs were determined by qRT‐PCR. (H) RAW264.7 cells were transfected with FLAG‐Vector or FLAG‐OTUB2 plasmids for 24 h. Thereafter, cells were lysed and analysed by Western blot with indicated antibodies. (I) After treatment with 20 ng/mL CHX for indicated periods of time, BMDMs isolated from Otub2 +/+ and Otub2 –/– mice were lysed and analysed by Western blot with indicated antibodies. (J, K) BMDMs isolated from Otub2 +/+ and Otub2 –/– mice were treated with 20 µM MG132 (J) or 50 µM CQ (K) for 6 h or left untreated. Whole Cell lysates were analysed by Western blot with indicated antibodies. Representative immunoblots (upper panel) and quantification (lower panel) are shown. Data in D–G, J and K are shown as mean ± SEM. ns, no significant difference. * p < .05, ** p < .01.

Techniques: Derivative Assay, Western Blot, Quantitative Proteomics, Quantitative RT-PCR, Transfection, Plasmid Preparation, Isolation

OTUB2 interacts with RIPK2 to mediate K48 deubiquitination. (A) BMDM lysates were immunoprecipitated with anti‐OTUB2 antibody. Immunoprecipitated proteins and cell lysates were analysed by Western blot with indicated antibodies. (B) Subcellular distribution of OTUB2 (red) and RIPK2 (green) in BMDMs was examined by immunofluorescence. Scale bar = 5 µm. (C, D) NIH/3T3 cells were co‐transfected with FLAG‐OTUB2 and HIS‐MYC‐RIPK2 plasmids for 24 h. Whole‐cell lysates were immunoprecipitated with anti‐FLAG (C) or anti‐MYC (D) antibodies. Immunoprecipitated proteins and cell lysates were analysed by Western blot with the indicated antibodies. (E) BMDMs were treated with 20 µM MG132 for 6 h before lysis. Proteins were immunoprecipitated from whole‐cell lysates with anti‐RIPK2 antibody and analysed by Western blot. (F, G) Schematic diagram (F) and representative immunoblots (G) of the in vitro deubiquitination assay. (H) Schematic diagram of the OTUB2 active site and the C51S mutant construct. (I, J) NIH/3T3 cells were transfected with the indicated plasmids for 24 h, followed by treatment with 20 µM MG132 for 6 h. Proteins were immunoprecipitated from whole‐cell lysates with anti‐MYC antibody and analysed by Western blot. (K) NIH/3T3 cells were transfected with the indicated plasmids for 24 h. Whole‐cell lysates were analysed by Western blot with the indicated antibodies.

Journal: Clinical and Translational Medicine

Article Title: Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation

doi: 10.1002/ctm2.70038

Figure Lengend Snippet: OTUB2 interacts with RIPK2 to mediate K48 deubiquitination. (A) BMDM lysates were immunoprecipitated with anti‐OTUB2 antibody. Immunoprecipitated proteins and cell lysates were analysed by Western blot with indicated antibodies. (B) Subcellular distribution of OTUB2 (red) and RIPK2 (green) in BMDMs was examined by immunofluorescence. Scale bar = 5 µm. (C, D) NIH/3T3 cells were co‐transfected with FLAG‐OTUB2 and HIS‐MYC‐RIPK2 plasmids for 24 h. Whole‐cell lysates were immunoprecipitated with anti‐FLAG (C) or anti‐MYC (D) antibodies. Immunoprecipitated proteins and cell lysates were analysed by Western blot with the indicated antibodies. (E) BMDMs were treated with 20 µM MG132 for 6 h before lysis. Proteins were immunoprecipitated from whole‐cell lysates with anti‐RIPK2 antibody and analysed by Western blot. (F, G) Schematic diagram (F) and representative immunoblots (G) of the in vitro deubiquitination assay. (H) Schematic diagram of the OTUB2 active site and the C51S mutant construct. (I, J) NIH/3T3 cells were transfected with the indicated plasmids for 24 h, followed by treatment with 20 µM MG132 for 6 h. Proteins were immunoprecipitated from whole‐cell lysates with anti‐MYC antibody and analysed by Western blot. (K) NIH/3T3 cells were transfected with the indicated plasmids for 24 h. Whole‐cell lysates were analysed by Western blot with the indicated antibodies.

Techniques: Immunoprecipitation, Western Blot, Immunofluorescence, Transfection, Lysis, In Vitro, Mutagenesis, Construct

MDP treatment alleviates DSS‐induced colitis in Otub2 +/+ mice but not in Otub2 –/– mice. (A) Otub2 +/+ and Otub2 –/– mice were fed drinking water containing 2% DSS for 7 days and subsequently given regular drinking water for 3 days. In addition, mice were intraperitoneally injected with either MDP (100 µg; 200 µL/mouse) or 200 µL of PBS on days 0, 1 and 2. Body weight (A) and disease activity index (B) were recorded daily ( n = 6/group). (C, D) The representative image (C) and length (D) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. (E, F) Histology score (E) as well as representative H&E and PAS/AB staining (F) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. Scale bar = 50 µm. Data in A, B, D and E are shown as mean ± SEM. ns, no significant difference. * p < .05, ** p < .01, *** p < .001.

Journal: Clinical and Translational Medicine

Article Title: Deubiquitination of RIPK2 by OTUB2 augments NOD2 signalling and protective effects in intestinal inflammation

doi: 10.1002/ctm2.70038

Figure Lengend Snippet: MDP treatment alleviates DSS‐induced colitis in Otub2 +/+ mice but not in Otub2 –/– mice. (A) Otub2 +/+ and Otub2 –/– mice were fed drinking water containing 2% DSS for 7 days and subsequently given regular drinking water for 3 days. In addition, mice were intraperitoneally injected with either MDP (100 µg; 200 µL/mouse) or 200 µL of PBS on days 0, 1 and 2. Body weight (A) and disease activity index (B) were recorded daily ( n = 6/group). (C, D) The representative image (C) and length (D) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. (E, F) Histology score (E) as well as representative H&E and PAS/AB staining (F) of colons from Otub2 +/+ and Otub2 –/– mice on day 10 after DSS treatment. Scale bar = 50 µm. Data in A, B, D and E are shown as mean ± SEM. ns, no significant difference. * p < .05, ** p < .01, *** p < .001.

Techniques: Injection, Activity Assay, Staining

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